Introduction
The proteome is the full set of proteins being produced by a cell from what is encoded in the genome [1,3]. The proteome is dynamic and changes over time [3]. Studying the proteome involves studying how proteins interact with one another to carry out biological processes [1]. Proteins often associate with one another to carry out their function and studying their interactions helps shed light on the exact details of cellular pathways.
Results
Discussion
The larger protein interaction network shows the high degree of similarity in interactions between the human and mouse RAB27A. This high degree of similarity gives further confidence that mice are an effective model organism for studying GS and that mouse findings will be to some degree applicable to humans.
The smaller interaction network color coded to biological process shows some interesting results. We see that in humans and mice RAB27A is involved in similar biological processes. Additionally, we see that in both species the protein forms a complex with MYO5A and MLPH. MYO5A is the gene who's mutations are implicated in Griscelli Syndrome Type 1. It seems very interesting that the genes implicated in multiple types of the disease are interacting with one another and yet have both common and separate symptoms. If that same complex is still formed in RAB27A mutants and if those proteins are still interacting is of interest to studying GS.
The smaller interaction network color coded to biological process shows some interesting results. We see that in humans and mice RAB27A is involved in similar biological processes. Additionally, we see that in both species the protein forms a complex with MYO5A and MLPH. MYO5A is the gene who's mutations are implicated in Griscelli Syndrome Type 1. It seems very interesting that the genes implicated in multiple types of the disease are interacting with one another and yet have both common and separate symptoms. If that same complex is still formed in RAB27A mutants and if those proteins are still interacting is of interest to studying GS.
References
[1]“Proteome.” Nature News, Nature Publishing Group, www.nature.com/scitable/definition/proteome-297/.
[2]“Welcome to STRING.” STRING, STRING Consortium, string-db.org/.
[3]“What Is Proteomics?” EMBL-EBI Train Online, 12 Sept. 2018, www.ebi.ac.uk/training/online/course/proteomics-introduction-ebi-resources/what-proteomics.
[1]“Proteome.” Nature News, Nature Publishing Group, www.nature.com/scitable/definition/proteome-297/.
[2]“Welcome to STRING.” STRING, STRING Consortium, string-db.org/.
[3]“What Is Proteomics?” EMBL-EBI Train Online, 12 Sept. 2018, www.ebi.ac.uk/training/online/course/proteomics-introduction-ebi-resources/what-proteomics.
The web page was produced as an assignment for Genetics 564, an undergraduate capstone course at UW-Madison